May 25, 2017 | Characterization, Glossary of Spectroscopy Terms
Accuracy is a general term that describes the agreement between a measurement and a true value. Precision is a general term that describes how close measured values are to each other, if they are clustered or spread out. Accuracy is affected by both random and...
May 25, 2017 | Glossary of Spectroscopy Terms, Software
To increase S/N, several acquisitions can be averaged together. This is called time averaging. Many sources of noise are random, meaning that they cause deviations around a true value. This means that the average is a statistical estimator for the true value. The...
May 25, 2017 | Glossary of Spectroscopy Terms, Sampling
A blank is a sample that contains everything except for the analyte of interest. For example, if you are doing a UV-vis experiment to measure concentrations of Green Fluorescent Protein, the protein has to be dissolved in a solvent. The blank is a sample of just the...
May 25, 2017 | Detectors, Glossary of Spectroscopy Terms
There are 15 “optically black” pixels on StellarNet detectors that are not hit by light during an acquisition. They provide a continuous measurement of the average dark spectrum and can be used to adjust for baseline drift during an experiment. In other words, this...
May 25, 2017 | Glossary of Spectroscopy Terms, Optics, Spectrograph
Stray light is any light that hits the detector unintentionally. The detector is not able to distinguish wavelengths, so it cannot tell the difference between the e.g. 500 nm light that is supposed to hit a pixel and the 505 nm light that accidentally hit it. It will...
May 25, 2017 | Detectors, Glossary of Spectroscopy Terms
Electronic noise is all the noise generated by the electronic circuits after the electrons are converted to a voltage. This includes noise from any amplifiers and the A/D converter. For example, conversions of exactly the same charge will not necessarily yield exactly...